Day: March 21, 2022

´╗┐Immunoblotting was performed with anti-Zscan4 antibodies initial. Oct-4 antibody. B. (i) Localisation of Zscan4c-V5-His proteins was evaluated by immunostaining of Tet-off inducible cell lines expanded in the lack of tetracycline. Anti-V5 antibody was utilized to identify Zscan4c-V5 proteins (reddish colored). Cell nuclei had been counter-stained Apronal with DAPI (blue). (ii) Zscan4c-V5-His-Tet-off inducible mESC (clone 45) […]