Day: July 26, 2022

C) Prohibitin was identified by mass spectrometry American blotting detection Traditional western blotting was performed to detect the autoantibodies in sufferers’ sera of RA. pET-28a(+). Proteins was over-expressed in the BL21 (DE), accompanied by the purification of recombinant protein using Ni-NTA resin (Qiagen, Hilden, Germany). The concentrations of proteins had been dependant on BCA package […]