The eluted fractions were combined and buffer-exchanged with 50 mm Tris-Cl, pH 8.0, 200 mm NaCl, and 10% glycerol using 10,000 kDa cut-off centrifugal filters (Millipore). ER-binding components, Pin1 improves the binding affinity of ER to consensus DNA components selectively. These research reveal that Pin1 isomerization of phosphorylated ER can straight regulate the function from […]